Review




Structured Review

TransGen biotech co plasmids pvax1 chxcl1 emef1α
Plasmids Pvax1 Chxcl1 Emef1α, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 96/100, based on 351 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transintrotm+el+transfection+reagent/TransIntro+EL+Transfection+Reagent/pm41976087-59-13-20
Average 96 stars, based on 351 article reviews
plasmids pvax1 chxcl1 emef1α - by Bioz Stars, 2026-10
96/100 stars

Images

Related Articles

Transfection:

Article Title: DNA large fragment deleting by compact, sequence-motif-free and specific TaqTth-hpRNA assisted with the microhomology-mediated end joining pathway
Article Snippet: .. The following day, each well was transfected with 3 μl TransIntroTM EL Transfection Reagent (TransGen Biotech, #FT201-01), 600 ng of pcDNA3.1(+)-HA-NLS-TaqTth plasmid, and 400 ng of hpRNA plasmid (200 ng of hpRNA-S and 200 ng of hpRNA-A) following the manufacturer’s recommended protocol. ..

Article Title: Molecular characterization and functional analysis of buffalo prolactin (PRL) in mammary gland development and lactation
Article Snippet: One correct clone was selected, and the EndoFree Plasmid Kit (Tiangen, Beijing, China) was used to extract the plasmid for subsequent transfection, with a final concentration of ≥ 1 μg μL−1. .. When cells reached approximately 70 %–80 % confluence in six-well plates, 3 μg of pEGFP-N1-PRL plasmid or 3 μg of pEGFP-N1 empty vector was transiently transfected into BuMECs using TransIntroTM EL transfection reagent (TransGen Biotech, Beijing, China) according to the manufacturer’s instructions. ..

Article Title: Cyprinid herpesvirus 2 ORF41 Protein Degrades Pyruvate Dehydrogenase (PDH)-E1β to Promote Viral Replication in Gibel Carp Brain (GiCB) Cells
Article Snippet: .. Plasmid DNA from validated clones (confirmed by Sanger sequencing) was transfected into GiCB cells using TransIntroTM EL Transfection Reagent (TransGen Biotech, Beijing, China) at a 1:2 plasmid DNA-to-reagent ratio following a 15 min incubation. ..

Article Title: GAS41 promotes ITGA4-mediated PI3K/Akt/mTOR signaling pathway and glioma tumorigenesis.
Article Snippet: Glioma Amplified Sequence 41 (GAS41) is a chromatin-associated protein that belongs to the YEATS domain family of proteins and is frequently amplified in various tumors.. However, its biological function and carcinogenic mechanism in gliomas are not fully understood.. In this study, we revealed that GAS41 was upregulated in human glioma tissues and cell lines, and higher expression of GAS41 was significantly associated with

Article Title: CiLSM14Aa acts as a cytosolic dsRNA sensor to activate antiviral immunity against GCRV-II.
Article Snippet: The innate immune system relies on pattern recognition receptors (PRRs) to detect viral pathogens and initiate antiviral responses.. LSM14A, a conserved RNA-binding protein involved in mRNA metabolism, has recently emerged as a cytosolic nucleic acid sensor in vertebrates.. However, its role in teleost immunity remains unclear.

Article Title: Evaluation of Immunoprotective Effects of DNA Vaccine Based on Eimeria maxima EF-1α Antigen and Chicken XCL1 Chemokine
Article Snippet: .. Briefly, plasmids (1 μg; pVAX1-ChXCL1, pVAX1-EmEF1α, pVAX1-ChXCL1-EmEF1α, or the empty pVAX1 vector control) and TransIntroTM EL Transfection Reagent (2 μL; TransGen Biotech Co., Ltd., Beijing, China) were separately diluted in 50 μL aliquots of opti-MEM ® medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). ..

Article Title: MiR-130c-5p targets the SHVV n gene and upregulates immune cytokines (IL-6, IL-22, IL-1β) to inhibit viral replication
Article Snippet: .. CCO cells were co-transfected with 30 pmol of NC mimic, miR-130c-5p mimic, NC inhibitor or miR-130c-5p inhibitor with 500 ng of luciferase reporter plasmids pmirGLO-N and pmirGLO-N mut using TransIntroTM EL transfection reagent (TransGen Biotech, China). co-transfected CCO cells. .. Twenty-four hours after transfection, luciferase activity was measured using the Dual-Glo luciferase assay system (Promega, USA).

Plasmid Preparation:

Article Title: DNA large fragment deleting by compact, sequence-motif-free and specific TaqTth-hpRNA assisted with the microhomology-mediated end joining pathway
Article Snippet: .. The following day, each well was transfected with 3 μl TransIntroTM EL Transfection Reagent (TransGen Biotech, #FT201-01), 600 ng of pcDNA3.1(+)-HA-NLS-TaqTth plasmid, and 400 ng of hpRNA plasmid (200 ng of hpRNA-S and 200 ng of hpRNA-A) following the manufacturer’s recommended protocol. ..

Article Title: Molecular characterization and functional analysis of buffalo prolactin (PRL) in mammary gland development and lactation
Article Snippet: One correct clone was selected, and the EndoFree Plasmid Kit (Tiangen, Beijing, China) was used to extract the plasmid for subsequent transfection, with a final concentration of ≥ 1 μg μL−1. .. When cells reached approximately 70 %–80 % confluence in six-well plates, 3 μg of pEGFP-N1-PRL plasmid or 3 μg of pEGFP-N1 empty vector was transiently transfected into BuMECs using TransIntroTM EL transfection reagent (TransGen Biotech, Beijing, China) according to the manufacturer’s instructions. ..

Article Title: Cyprinid herpesvirus 2 ORF41 Protein Degrades Pyruvate Dehydrogenase (PDH)-E1β to Promote Viral Replication in Gibel Carp Brain (GiCB) Cells
Article Snippet: .. Plasmid DNA from validated clones (confirmed by Sanger sequencing) was transfected into GiCB cells using TransIntroTM EL Transfection Reagent (TransGen Biotech, Beijing, China) at a 1:2 plasmid DNA-to-reagent ratio following a 15 min incubation. ..

Quantitative RT-PCR:

Article Title: ADAMTS13 attenuates renal fibrosis by suppressing thrombospondin 1 mediated TGF-β1/Smad3 activation.
Article Snippet: Renal fibrosis is a common pathologic pathway for the progression of chronic kidney disease (CKD) to end-stage renal disease (ESRD).. Its mechanisms are unclear and it lacks effective therapy.. Thrombospondin 1 (TSP1) mediated transforming growth factor-β1 (TGF-β1) activation was confirmed to promote renal fibrosis.

Western Blot:

Article Title: ADAMTS13 attenuates renal fibrosis by suppressing thrombospondin 1 mediated TGF-β1/Smad3 activation.
Article Snippet: Renal fibrosis is a common pathologic pathway for the progression of chronic kidney disease (CKD) to end-stage renal disease (ESRD).. Its mechanisms are unclear and it lacks effective therapy.. Thrombospondin 1 (TSP1) mediated transforming growth factor-β1 (TGF-β1) activation was confirmed to promote renal fibrosis.

Clone Assay:

Article Title: Cyprinid herpesvirus 2 ORF41 Protein Degrades Pyruvate Dehydrogenase (PDH)-E1β to Promote Viral Replication in Gibel Carp Brain (GiCB) Cells
Article Snippet: .. Plasmid DNA from validated clones (confirmed by Sanger sequencing) was transfected into GiCB cells using TransIntroTM EL Transfection Reagent (TransGen Biotech, Beijing, China) at a 1:2 plasmid DNA-to-reagent ratio following a 15 min incubation. ..

Sequencing:

Article Title: Cyprinid herpesvirus 2 ORF41 Protein Degrades Pyruvate Dehydrogenase (PDH)-E1β to Promote Viral Replication in Gibel Carp Brain (GiCB) Cells
Article Snippet: .. Plasmid DNA from validated clones (confirmed by Sanger sequencing) was transfected into GiCB cells using TransIntroTM EL Transfection Reagent (TransGen Biotech, Beijing, China) at a 1:2 plasmid DNA-to-reagent ratio following a 15 min incubation. ..

Incubation:

Article Title: Cyprinid herpesvirus 2 ORF41 Protein Degrades Pyruvate Dehydrogenase (PDH)-E1β to Promote Viral Replication in Gibel Carp Brain (GiCB) Cells
Article Snippet: .. Plasmid DNA from validated clones (confirmed by Sanger sequencing) was transfected into GiCB cells using TransIntroTM EL Transfection Reagent (TransGen Biotech, Beijing, China) at a 1:2 plasmid DNA-to-reagent ratio following a 15 min incubation. ..

Control:

Article Title: Evaluation of Immunoprotective Effects of DNA Vaccine Based on Eimeria maxima EF-1α Antigen and Chicken XCL1 Chemokine
Article Snippet: .. Briefly, plasmids (1 μg; pVAX1-ChXCL1, pVAX1-EmEF1α, pVAX1-ChXCL1-EmEF1α, or the empty pVAX1 vector control) and TransIntroTM EL Transfection Reagent (2 μL; TransGen Biotech Co., Ltd., Beijing, China) were separately diluted in 50 μL aliquots of opti-MEM ® medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). ..

Luciferase:

Article Title: MiR-130c-5p targets the SHVV n gene and upregulates immune cytokines (IL-6, IL-22, IL-1β) to inhibit viral replication
Article Snippet: .. CCO cells were co-transfected with 30 pmol of NC mimic, miR-130c-5p mimic, NC inhibitor or miR-130c-5p inhibitor with 500 ng of luciferase reporter plasmids pmirGLO-N and pmirGLO-N mut using TransIntroTM EL transfection reagent (TransGen Biotech, China). co-transfected CCO cells. .. Twenty-four hours after transfection, luciferase activity was measured using the Dual-Glo luciferase assay system (Promega, USA).



Similar Products

96
TransGen biotech co plasmids pvax1 chxcl1 emef1α
Plasmids Pvax1 Chxcl1 Emef1α, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transintrotm+el+transfection+reagent/TransIntro+EL+Transfection+Reagent/pm41976087-59-13-20
Average 96 stars, based on 1 article reviews
plasmids pvax1 chxcl1 emef1α - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

96
TransGen biotech co transintrotm el transfection reagent
Transintrotm El Transfection Reagent, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transintrotm+el+transfection+reagent/TransIntro+EL+Transfection+Reagent/pmc13072396-69-14-20
Average 96 stars, based on 1 article reviews
transintrotm el transfection reagent - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

93
TransGen biotech co transintrotm
Transintrotm, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transintrotm+el+transfection+reagent/TransIntro+EL+Transfection+Reagent+-+2+%C3%97/pm39341378-50-8-10
Average 93 stars, based on 1 article reviews
transintrotm - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier

Image Search Results